BIOT125 Biotechnology Methods and Techniques

Department of Science, Technology, Engineering & Mathematics: Biotechnology

I. Course Number and Title
BIOT125 Biotechnology Methods and Techniques
II. Number of Credits
4 credits
III. Number of Instructional Minutes
IV. Prerequisites
CHEM121 (C or better)
V. Other Pertinent Information
VI. Catalog Course Description
This course is an introduction to common practices and methodologies of Biotechnology. Students receive hands-on experience using and maintaining laboratory instruments. Laboratory procedures include: solution preparation, aseptic technique, protein separations and assays, DNA electrophoresis, and Polymerase Chain Reaction (PCR). Lectures support the laboratory activities.
VII. Required Course Content and Direction
  1. Course Learning Goals

    Students will:

    1. recognize and employ laboratory safety practices when using biological and chemical materials;
    2. prepare and manage accurate laboratory notebooks and instrument logs;
    3. operate, calibrate, and maintain standard equipment found in a biotechnology laboratory;
    4. define and perform common laboratory measurements using micropippetors and volumetric apparati;
    5. explain and perform protein detection and quantification assays; and
    6. practice basic DNA procedures, including restriction digestion and gel electrophoresis.
  2. Planned Sequence of Topics and/or Learning Activities

    1. introduction to the Biotechnology Lab
    2. laboratory safety and documentation
    3. laboratory measurements and math
    4. solution and media preparation
    5. aseptic techniques
    6. spectrophotometry and graphing
    7. filtration and centrifugation
    8. protein detection and quantification
    9. DNA digestion and electrophoresis
    10. Polymerase Chain Reaction
    11. Student Project Presentations
  3. Assessment Methods for Course Learning Goals

    Student progress and achievement is assessed through lab reports, project presentations, worksheets, take-home research assignments, and in-class exams.
  4. Reference, Resource, or Learning Materials to be used by Student:

    Students use educational resources as approved by the department and specified in the individual instructor's syllabus.

Review/Approval Date - 4/09; New Core 8/2015