BIOT205 Cell and Tissue Culture
Department of Science, Technology, Engineering & Mathematics: Biotechnology
Course Number and TitleBIOT205 Cell and Tissue Culture
Number of Credits4 credits
Minimum Number of Instructional Minutes Per Semester4500 minutes
PrerequisitesBIOT125 (C or better) and BIOL121 (C or better)
Other Pertinent InformationNone
Catalog Course DescriptionThis course is an introduction to the theory, standard practices, and methodologies of animal cell culture. Students will get hands-on laboratory experience including sterile technique, media preparation, cell counting, maintenance and storage of cell lines, and scale-up. Lectures will support the laboratory activities.
Required Course Content and Direction
- Course Learning Goals
- recognize and employ biosafety guidelines and practices;
- operate, calibrate, and maintain standard equipment found in an animal cell culture laboratory;
- prepare and sterilize media and solutions used in cell culture;
- grow, maintain, and store animal cell lines;
- characterize cells and cell products using standard techniques;
- perform scale-up of an animal cell culture.
- Core Learning Goals (if applicable)
Students will be able to
Planned Sequence of Topics and/or Learning Activities:
- Introduction to Cell and Tissue Culture
- Laboratory Safety and Documentation
- Aseptic Techniques
- Media Preparation and Sterilization
- Cell Counting Methods
- Growth of Adherent and Suspension Cultures
- Subculturing Techniques
- Maintenance and Cryopreservation of Cell Lines
- Mycoplasma and Virus Testing
- Cell Transfection
- Characterization of Cells and Cell Products
- Scale-up of Adherent and Suspension Cultures
Assessment Methods for Core Learning Goals:
- Assessment Methods for Course Learning Goals
- Assessment Methods for Core Learning Goals (if applicable)
Lab reports, project presentations, worksheets, take-home research assignments, and in-class exams will be used to assess student progress and success.
Reference, Resource, or Learning Materials to be used by Students:Students use educational resources as approved by the department and specified in the individual instructor's format.
Teaching Methods EmployedSection VIII is not being used in new and revised syllabi as of 12/10/08.
Review/Approval Date - 4/09