BIOT205 Cell and Tissue Culture
Department of Science, Technology, Engineering & Mathematics: Biotechnology
- I. Course Number and Title
- BIOT205 Cell and Tissue Culture Course No Longer Offered
- II. Number of Credits
- 4 credits
- III. Number of Instructional Minutes
- 4500
- IV. Prerequisites
- BIOT125 (C or better) and BIOL121 (C or better)
- Corequisites
- None
- V. Other Pertinent Information
- None
- VI. Catalog Course Description
- This course is an introduction to the theory, standard practices, and methodologies of animal cell culture. Students receive hands-on laboratory experience including sterile technique, media preparation, cell counting, maintenance and storage of cell lines, and scale-up. Lectures support the laboratory activities.
- VII. Required Course Content and Direction
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Course Learning Goals
Students will:
- recognize and employ biosafety guidelines and practices;
- operate, calibrate, and maintain standard equipment found in an animal cell culture laboratory;
- prepare and sterilize media and solutions used in cell culture;
- grow, maintain, and store animal cell lines;
- characterize cells and cell products using standard techniques; and
- perform scale-up of an animal cell culture.
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Planned Sequence of Topics and/or Learning Activities
- introduction to cell and tissue culture
- laboratory safety and documentation
- aseptic techniques
- media preparation and sterilization
- cell counting methods
- growth of adherent and suspension cultures
- subculturing techniques
- maintenance and cryopreservation of cell kines
- mycoplasma and virus testing
- cell transfection
- characterization of cells and cell products
- scale-up of adherent and suspension cultures
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Assessment Methods for Course Learning Goals
Lab reports, project presentations, worksheets, take-home research assignments, and in-class exams are used to assess student progress and success. -
Reference, Resource, or Learning Materials to be used by Student:
Students use educational resources as approved by the department and specified in the individual instructor's syllabus.
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Review/Approval Date - 4/09