BIOT205 Cell and Tissue Culture

Department of Science, Technology, Engineering & Mathematics: Biotechnology

I. Course Number and Title
BIOT205 Cell and Tissue Culture
II. Number of Credits
4 credits
III. Number of Instructional Minutes
IV. Prerequisites
BIOT125 (C or better) and BIOL121 (C or better)
V. Other Pertinent Information
VI. Catalog Course Description
This course is an introduction to the theory, standard practices, and methodologies of animal cell culture. Students receive hands-on laboratory experience including sterile technique, media preparation, cell counting, maintenance and storage of cell lines, and scale-up. Lectures support the laboratory activities.
VII. Required Course Content and Direction
  1. Course Learning Goals

    Students will:

    1. recognize and employ biosafety guidelines and practices;
    2. operate, calibrate, and maintain standard equipment found in an animal cell culture laboratory;
    3. prepare and sterilize media and solutions used in cell culture;
    4. grow, maintain, and store animal cell lines;
    5. characterize cells and cell products using standard techniques; and
    6. perform scale-up of an animal cell culture.
  2. Planned Sequence of Topics and/or Learning Activities

    1. introduction to cell and tissue culture
    2. laboratory safety and documentation
    3. aseptic techniques
    4. media preparation and sterilization
    5. cell counting methods
    6. growth of adherent and suspension cultures
    7. subculturing techniques
    8. maintenance and cryopreservation of cell kines
    9. mycoplasma and virus testing
    10. cell transfection
    11. characterization of cells and cell products
    12. scale-up of adherent and suspension cultures
  3. Assessment Methods for Course Learning Goals

    Lab reports, project presentations, worksheets, take-home research assignments, and in-class exams are used to assess student progress and success.
  4. Reference, Resource, or Learning Materials to be used by Student:

    Students use educational resources as approved by the department and specified in the individual instructor's syllabus.

Review/Approval Date - 4/09